Synonym:TSKgel®HydrophobicInteractionChromatography(HIC)ColumnProperties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,HydrophobicInteraction(HIC)Columns,TSKgelBioAssistPEEKHPLCColumns, | 
| material | PEEKhardware | 
| mfr.no. | Tosoh,20023 | 
| parameter | ≤4Msaltconcentration | 
|  | ≤50%organicsolvent | 
|  | 1.2mL/minflowrate | 
|  | 10-50°Ctemperature | 
|  | 20barmax.pressure | 
| application(s) | HPLC:suitable | 
| L×I.D. | 5 cm×7.8 mm | 
| matrix | polymerparticleplatform | 
| matrixactivegroup | phenylphase | 
| particlesize | 10 μm | 
| poresize | 1000 ? | 
| operatingpH | 2-12 | 
| modeofchromatography | Hydrophobicinteraction(HIC) | 
Description:
Generaldescription
Bothhydrophobicinteractionchromatography(HIC)andreversed-phaseliquidchromatographyseparateonthebasisofproteinhydrophobicityandallowselectivebindinganddesorptionofproteins.However,HICoperatesatsignificantlylowerbindingenergyandusesaqueousmobilephases.Thesecharacteristicsprovideagentletechniquethatislesslikelytodisturbproteinconformation.Asaresult,HICgenerallyprovidesbetteractivityrecovery.
TSKgelBioAssistPhenylcolumnscontainthesameparticlesasPhenyl-5PWcolumns,butarepackedinPEEKcolumnhardwareratherthanstainlesssteel.BioAsistPhenyl(Phenyl-5PW),TSKgelEther-5PWandButyl-NPRresin-basedcolumnsprovidearangeofhydrophobicitiesforchromatographicoptimization.Ammoniumsulfateconcentrationcanbeminimizedbyusingamorehydrophobicpacking,suchasBioAssistPhenyl.WerecommendanEther-5PWcolumnforpurifyingveryhydrophobicproteins.TSKgelBioAssistPhenylandTSKgelEther-5PWpackingsarebasedonTSKgelG5000PWresin-10??μmparticleswith1000???…pores.TSKgelButyl-NPRcolumns,whicharelesshydrophobicthanEther-5PW,ispreparedfrom2.5??μmnonporousparticles,allowingforrapidanalysesandsemi-micropreparativeapplications.Allthreecolumntypescanbecleanedwith0.2??MNaOH.Scale-upcanbeperformedbyusingcorrespondingToyopearlbulkresins.
ThemosthydrophobicamongthethreeTSKgelHICcolumns,TSKgelPhenyl-5PWisanexcellentchoicetoscreenfortheselectivity,retentionandrecoveryofmostbiomolecules.Featuringa1000?poresizeinapolymethacrylatebasematerial,thePhenyl-5PWcolumnisstableineitheracidorcausticcleaningregimensandprovidesexcellentaccesstolargermoleculeswithlowdiffusioncoefficients.StainlesssteelisthestandardcolumnformatforTSKgelPhenyl-5PW.GlassandPEEKcolumnhousingsareavailableforsamplesthatmayadsorbtostainlesssteel.Thealternativecolumnformatsarealsosuitableforgeneralapplications.
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LegalInformation
BioAssistisaregisteredtrademarkofTosohCorporation
TSKgelisaregisteredtrademarkofTosohCorporation