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Millipore/16-213 | Anti-Myc Tag Antibody, clone 4A6, HRP conjugate/16-213/100 µL
  • Millipore/16-213 | Anti-Myc Tag Antibody, clone 4A6, HRP conjugate/16-213/100 µL

Millipore/16-213 | Anti-Myc Tag Antibody, clone 4A6, HRP conjugate/16-213/100 µL

價(jià)格: ￥4440.00 市場(chǎng)價(jià): 7400.00
貨號(hào): 16-213
品牌: Millipore
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    • Description
      Catalogue Number16-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 4A6, HRP conjugate
      OverviewImmunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

      Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

      Species Cross-reactivity: Human, others not tested

      Ig Type: IgG

      Host: Mouse

      Molecular Weight: Varies depending upon the protein which is tagged
      Alternate Names
      • Transcription factor p64
      • avian myelocytomatosis viral oncogene homolog
      • myc proto-oncogene protein
      • v-myc avian myelocytomatosis viral oncogene homolog
      • v-myc myelocytomatosis viral oncogene homolog (avian)
      Background InformationMyc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.
      Product Information
      FormatHRP
      Control
      • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
      PresentationPurified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon?, 0.2 mg/mL BSA) and 50% glycerol.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20oC from date of receipt.
      Applications
      ApplicationAnti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.
      EpitopeC-terminal
      Clone4A6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Other species cross-reactivity not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular WeightVaries depending upon the protein which is tagged.
      Physicochemical Information
      Dimensions
      Materials Information
      Materials Information
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