| Immunogen | Syntheticpeptide(C-KQLLHGEPNVS[pY]ICSRY)correspondingtoaminoacids203-219derivedfromaregionofthecatalyticdomain(betweensuBDomainVIIandVIII)oftheDrosophilaGSK3/shaggyenzymecoupledtoovalbumin. |
| Epitope | SurroundingpTyr214(DrosophilaGSK-3),pTyr279(humanGSK3α),pTyr216(humanGSK3β) |
| Clone | 5G-2F |
| Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
| Host | Mouse |
| Specificity | RecognizesGSK-3phosphorylatedatthetargettyrosineresidue(humanGSK-3αpY279andGSK-3βpY216).The17-aminoacidimmunogenpeptidesequencediffersfromthecorrespondinghuman,mouse,andratsequencebyonlytwoaminoacidresidues. |
| Isotype | IgG1κ |
| SpeciesReactivity | |
| SpeciesReactivityNote | Human,mouse,andrat.PredictedtoreactwithGSK-3orthologsfromdrosophilamelanogasterandmanyotherspeciesbasedonsequencehomologysurroundingthetargetphosphotyrosineresidue. |
| AntibodyType | MonoclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Glycogensynthasekinase3-alpha(GSK3A;EC{2.7.1.37})isamultifunctionalproteinserinekinase,homologoustoDrosophila"shaggy"(zeste-white3)andimplicatedinthecontrolofseveralregulatoryproteinsincludingglycogensynthase(seeGYS1,{138570})andtranscriptionfactors(e.g.,JUN,{165160}).ItalsoplaysaroleintheWNT({164820})andPI3K(seePIK3CG,{601232})signalingpathways(seereviewby{1:Alietal.(2001)}).[suppliedbyOMIM] |
| GeneSymbol | |
| Modifications | |
| PurificationMethod | ProteinGChromatography |
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:P49840#Implicatedinthehormonalcontrolofseveralregulatoryproteinsincludingglycogensynthase,MYBandthetranscriptionfactorJUN(Bysimilarity). SIZE:483aminoacids;50981Da SUBUNIT:Monomer(Bysimilarity). SIMILARITY:SwissProt:P49840##Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.GSK-3subfamily.&Contains1proteinkinasedomain. |
| MolecularWeight | 51kDa(alpha)and46kDa(beta) |